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KMID : 0357919710050010019
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Korean Journal of Pathology
1971 Volume.5 No. 1 p.19 ~ p.23
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A Rapid Micromethod for Determination of Serum Calcium by Means of Orthocresolphthalein Complexone
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김기홍(金箕洪)/Ki Hong Kim
이주섭(李周燮)/Joo Sup Lee
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Abstract
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This study was undertaken to evaluate the usefulness of a rapid micromethod for
determination of serum calcium.
In 1966 Connrty and Briggs presented a greatly improved manual spectrophotometric
method based upon release of the bound calcium by means of protein precipitation and
the complete masking of the response of the dye to magnesium by means of
8-quinolinol.
Their method was applied to a rapid micromethod in which the process of
deproteinization was omitted and only 50 microliters serum was used as a sample
instead of 1ml.
Clark-Collip method, Diehl-Ellingboe's EDTA method, and a micromethod using
orthocresolphthalein a colar regent were carried out with thirty samples to compare the
results each other. On the other hand normal values of healthy Koreans, male 96, female
94, were surveyed by the above mentioned micromethod.
The results were followings.
1. Clark-Collip method: mean 9.03 mg
Diehl-Ellingboe method: mean 8.99 mg
A rapid micromethod: mean 8.88 mg
2. Normal values of Koreans by the rapid micromethod: male 9.47±0.45 mg, female
8.79±0.41 mg%, without consideration of sex 9.14±0.53 mg%
Serum calcium levels of Koreans appeared to be lower than those of whites.
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